Conjugation of nucleotides with fluorescent dyes, biological tags, proteins, resins or nanomaterias provides acces to versatile nucleotide-based research tools for biophysics and molecular biology. Moreover, conjugation of nucleotides with appropriate nano(bio)materials may increase their stability and bioavailability which are one of the main obstacles limiting nucleotide therapeutic applications.
We are trying to combine our experience in nucleotide chemistry with (fluorescent/nano/bio)-material chemistry to obtain functionalized mRNA cap analogs and other biologically relevant nucleotides with new desired properties. Such compounds may become research tools, diagnostic tools or nucleotide-based therapeutics.
- The goals currently being pursued in our group concerning this rea of research are:
- – Synthesis and application of nucleotide based affinity resins.
- – Synthesis and application of fluorescently labelled or biotinylated nucleotides.
- – mRNA cap analog cellular delivery through conjugation with Adenovirus Dodecahedron or encapsulation in microvessels
- Magnetic-nanoparticle-decorated polypyrrole microvessels: Toward encapsulation of mRNA cap analogs, Krystyna Kijewska, Anita Jarzębińska, Joanna Kowalska, Jacek Jemielity, Daria Kępińska, Jacek Szczytko, Marcin Pisarek, Katarzyna Wiktorska, Jarosław Stolarski, Pawel G. Krysinski, Andrzej Twardowski, Maciej Mazur, Biomacromolecules 14 (2013) Just Accepted Manuscript, DOI: 10.1021/bm400250g
- Synthesis of biotin labelled cap analogue – incorporable into mRNA transcripts and promoting cap-dependent translation, Jemielity J., Lukaszewicz M., Kowalska J., Czarnecki J., Zuberek J., Darzynkiewicz E., Org. Biomol. Chem.10 (2012) 8570 – 8574
- Affinity resins containing enzymatically resistant mRNA cap analogs-a new tool for the analysis of cap-binding proteins, Szczepaniak S.A., Zuberek J., Darzynkiewicz E., Kufel J., Jemielity J. RNA 18 (2012) 1421 – 1432